Human IL-8 / CXCL8 Protein, His Tag

Source : 

Human IL-8, His Tag (IL8-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 28 - Ser 99 (Accession # P10145-1).

 

Molecule : IL-8

 

Synonyms : CXCL8,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1

 

Format : Powder

 

Category : Cytokines&Growth Factors

 

Accession : NP_000575.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 10.3 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 10.3 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

 

References :

(1) Hedges JC. 2000. Am J Respir Cell Mol Biol. 23(1):86-94.
(2) Van Damme J. 1990. Eur J Immunol. 20(9):2113-8.
(3) Hébert CA. 1990. J Immunol. 145(9):3033-40.