Human IL-4R / CD124 ELISA Kit

分子 : IL4R

 

抗原貨號 : 10402-H08H

 

產品介绍 : For the quantitative determination of Human IL-4R / CD124 concentration in serum and cell culture supernates.

 

線性範圍: 3.125-200

 

包被抗體類型 : rabbit mAb

 

檢測抗體類型 : rabbit mAb

 

靈敏度: 2.6

 

天然樣本測定含量: The average concentration of IL4R in 10 normal human serum is 995.21 +/- 185.18 pg/mL ranging from 742.10 to 1257.58 pg/mL. HepG2 cells (0.2 x 10E5 cells/mL) were cultured 3 days. An aliquot of the cell culture supernate was removed, assayed for levels of natural DLL1, and measured 12 pg/mL.

 

特異性 : This assay recognizes both recombinant and natural Human IL4R. The factors listed below were prepared at 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of human IL4 at 2 ng/mL in a mid-range recombinant
human IL4R control were assayed for interference. No significant interference was observed.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution