Human IL-35 (IL12A & EBI3 Heterodimer) Protein_CT040-H0323H

分子 : IL12A & IL27B

保存條件 : Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

蛋白構建: A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (NP_000873.2) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Met1-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

纯度: > 90 % as determined by SDS-PAGE

内毒素 : < 1.0 EU per μg of the protein as determined by the LAL method

預測N端 : Arg 23 & Arg 21

缓冲液 : Lyophilized from sterile PBS, pH 7.4.

NP號 : NP_000873.2 & NP_005746.2

氨基酸序列 : Met1-Ser219 & Met1-Lys229

標籤 : C-human IgG1-Fc & His & C-human IgG1-Fc & Flag