Human IL-33 Protein, His Tag

Source : 

Human IL-33, His Tag (IL3-H52H7) is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1).

 

Molecule : IL-33

 

Synonyms : IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2

 

Format : Powder

 

Category : Cytokines&Growth Factors

 

Accession : AAH47085.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 20.2 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Interleukin 33 (IL33) is known as C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, RP11-575C20.2,and is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.

 

References :

(1) Lingel A,et al.2009, Structure 17 (10): 1398–410.
(2) Dinarello, C.A., 2005, Immunity 23:461.