Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag

Source : 

Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (IL2-H4210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1 (IL12B) & P29459-1 (IL12A)).

 

Molecule : IL-12B & IL-12A

 

Synonyms : IL12,p70,Interleukin-12

 

Format : Powder

 

Category : Cytokines&Growth Factors

 

Accession : NP_002178.2 (IL12B) & NP_000873.2 (IL12A)

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 36.6 kDa (IL12B) and 23.8 kDa (IL12A)

 

Characteristics :

Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.6 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with polyhistidine tag at the C-terminus and subunit IL-12

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.

 

References :

(1) Kaliński P., et al., 1997, J. Immunol. 159 (1): 28–35.
(2) Hsieh CS., et al., 1993, Science 260 (5107): 547–9.
(3) Wang KS., et al., 2000, Blood 95 (10): 3183–90.