Human IL-12 (IL12A & IL12B Heterodimer) Protein

A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.