Human IL-12 (IL12A & IL12B Heterodimer) Protein, Biotinylated_CT011-H08H-B

分子 : IL12A & IL12B

保存條件 : Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

蛋白構建: A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. The purified protein was biotinylated in vitro.

纯度: > 95 % as determined by SDS-PAGE.

内毒素 : < 1.0 EU per μg protein as determined by the LAL method.

預測N端 : Arg 23 & Ile 23

缓冲液 : Lyophilized from sterile PBS, pH 7.4

NP號 : P29459.2 & NP_002178.2

氨基酸序列 : Met1-Ser219 & Met1-Ser328

標籤 : C-His