Human IkB alpha / NFKBIA ELISA Kit

分子 : NFKBIA

 

抗原貨號 : 12045-H07E

 

產品介绍 : For the quantitative determination of Human IkB alpha / NFKBIA concentration in cell lysate.

 

線性範圍: 12.5-800

 

包被抗體類型 : rabbit mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 3.6

 

天然樣本測定含量: The concentration of Human NFKBIA detected in Hela, HepG2 and Jurkat cell lysates were respectively 156.17 ng/mL, 146.00 ng/mL and 53.75 ng/mL.The total protein concentration of them were about 9.3 mg/mL, 8.5 mg/mL and 6mg/mL.

 

特異性 : This assay recognizes both recombinant and natural Human NFKBIA. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 :

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution