Human IgG4 Fc Protein, Tag Free (SPR verified)

Source : 

Human IgG4 Fc, Tag Free (SPR verified) (IG4-H5205) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 327 (Accession # P01861).

 

Molecule : IgG4 Fc

 

Synonyms : IGHG4

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : N/A

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 25.8 kDa

 

Characteristics :

This protein carries no "tag". The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 30-32 kDa under reducing (R) condition, and 28-30 kDa and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).

 

References :

(1) Frangione B., et al., 1969, Nature 221:145-148.
(2) Kristiansen T.Z., et al., 2004, Mol. Cell. Proteomics 3:715-728.
(3) Chen R., et al., 2009, J. Proteome Res. 8:651-661.