Human IGFBP3 / IBP3 Protein (His Tag)

Species : Human

Synonyms : BP-53,IBP3,IGFBP3

Host : HEK293 Cells

Purity :> 85 % as determined by SDS-PAGE

Activity : 1. Measured by its binding ability in a functional ELISA. Immobilized human IGF2 (Cat:13032-H24E) at 10 £gg/mL (100 £gl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 18 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized human IGF1 (Cat:10598-H24E) at 10 £gg/mL (100 £gl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 24 ng/mL.
3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 0.05-0.2 £gg/mL in the presence of 14 ng/mL human IGFII.