Human IGF1R / CD221 / CD221 ELISA Pair Set

分子 : IGF1R

 

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.

 

 

線性範圍 : 156.25-10000

 

包被抗體 : 0.5 mg/mL of mouse anti-IGF1R monoclonal antibody. Dilute to a working concentration of 1 μg/mL in CBS before coating. (Catalog: # 10164-MM07)

 

檢測抗體: Each vial contains 35 μg biotinylated rabbit anti-IGF1R polyclonal antibody. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. Dilute to a working concentration of 0.25 μg/mL in detection antibody dilution buffer before use.

 

標準品 : Each vial contains 140 ng of recombinant IGF1R. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 10 ng/mL is recommended.

 

生物素交聯HRP: 50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.

 

靈敏度 : The minimum detectable dose of human IGF1R / CD221 was determined to be approximately 156 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

特異性 :

 

檢測原理 : The human IGF1R / CD221 ELISA Pair Set is for the quantitative determination of human IGF1R / CD221.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IGF1R / CD221 coated on a 96-well plate. Standards and samples are added to the wells, and any IGF1R / CD221 present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-IGF1R / CD221 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of IGF1R / CD221 present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.

 

檢測限: 156 pg/ml

 

血清樣本檢測範圍 :