Human IGF-Binding Protein 1 (IGFBP1) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. SHBG in culture medium (Human endometrial stromal cell (HESC) and EDTA/Plasma has also been used (see published references). Other fluids may be adapted

KitContents

1. Anti-IGFBP coated strip plate (96 wells), Cat. #901 1 plate 2. IGFBP1 Std. A, 0.0 ug/L; 2 ml, Cat # 902 1 Vial 3. IGFBP1 Std. B, 1 ug/L; 0.5 ml, Cat # 903 1 Vial 4. IGFBP1 Std. C, 5.0 ug/L; 0.5 ml, Cat # 904 1 Vial 5. IGFBP1 Std. D, 30 ug/L; 0.5 ml, Cat # 905 1 Vial 6. IGFBP1 Std. E, 100 ug/L; 0.5 ml, Cat # 906E 1 Vial 7. IGFBP1 Std. F, 250 ug/L; 0.5 ml, Cat # 906F 1 Vial 8. Assay Buffer, 26 ml; Cat # 907 1 bottle 9. Anti-hIGFBP1-HRP Conjugate Conc., 250 ul, cat # 908 1 Vial 10. IGFBP1 Control serum, see vial for exact value 1 Vial 11. Wash buffer concentrate (10X solution), 50 ml Cat.#W-10 12. TMB substrate , 13 ml, Cat. # S-20 1 bottle 13. Stop solution (oxalic acid), Cat. # T-30 1 bottle 14. Instruction Manual, M-900 1

Form

Stds=0-250/ug/L; Sample=25 ul; Sensitivity= 0.4 ug/L; 70 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

The specificity of IGFBP-1 ELISA kit was determined by measuring interference from high concentrations of human placental Lactogen, HCG, human prolactin, and human AFP. These hormones had a minimal interference in the BP-1 assay (0.01% or less). No measurable interference was detected from IGBP-2-5 (5000-10000 ug/L). Human IGFBP-1 ELISA kit species crossreactivity with IGFBP-1 from other species (mouse, rat, and monkey) has not been established.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates