Human ICAM-1 / CD54 Protein, His Tag

Source : 

Human ICAM-1, His Tag (IC1-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Glu 480 (Accession # P05362-1).

 

Molecule : ICAM-1

 

Synonyms : ICAM1,BB2,CD54,P3.58

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : AAH15969.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 51.4 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 51.4 kDa. The protein migrates as 60-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Inter-Cellular Adhesion Molecule 1 (ICAM-1) is also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial cells and cells of the immune system. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies. ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds.

 

References :

(1) Rothlein, R. D., 1986, Journal of Immunology 137 (4): 1270–1274.
(2) Yang L, et al., 2005, Blood 106 (2): 584–92.
(3) Polin RS, et al., 1998, J. Neurosurg. 89 (4): 559–67.
(4) Frijns CJ, Kappelle LJ., 2002, Stroke 33 (8): 2115–22.