Human HMGB1 Protein, Mouse IgG2a Fc Tag, low endotoxin

Source : 

Human HMGB1, Mouse IgG2a Fc Tag, low endotoxin (HM1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Glu 215 (Accession # P09429-1).

 

Molecule : HMGB1

 

Synonyms : HMGB1,HMG1,HMG3,SBP-1

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_002119.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 51.6 kDa

 

Characteristics :

This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 51.6 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 0.1 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.

 

References :

(1) Wang H, et al., 1999, Science 285 (5425): 248–51.
(2) Yang H, et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107 (26): 11942–7.
(3) Yang H, et al., 2010, Biochim. Biophys. Acta 1799 (1-2): 149–56.
(4) Fagone P, et al., 2011, Gene Ther. 18 (11): 1070–7.