Human GUCY2C / Guanylyl cyclase C Protein, Fc Tag

Source : 

Human GUCY2C, Fc Tag (GUC-H5257) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).

 

Molecule : GUCY2C

 

Synonyms : GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_004954.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 72.5 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5

 

Description :

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.

 

References :

(1) Magee MS, et al. Cancer Immunol Res. 2018. 6(5):509-516.
(2) Li P, et al. Cancer Res. 2017. 77(18):5095-5106.
(3) Arshad N, et al. J Biol Chem. 2013. 288(6):3907-17.