Human Gremlin / GREM1 Protein, His Tag

Source : 

Human Gremlin, His Tag (GR1-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 184 (Accession # NP_037504).

 

Molecule : Gremlin

 

Synonyms : GREM1,CKTSF1B1,DAND2,DRM,PIG2

 

Format : Powder

 

Category : Cytokines&Growth Factors

 

Accession : NP_037504

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 20.2 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.2 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4 with 1 mM EDTA

 

Description :

Gremlin is also known as Cysteine knot superfamily 1, BMP antagonist 1 (CKTSF1B1), DAN domain family member 2 (DAND2), Down-regulated in Mos-transformed cells protein (DRM), Increased in high glucose protein 2 (IHG-2), Cell proliferation-inducing gene 2 protein (PIG2) or Gremlin-1 (GREM1), which is highly expressed in small intestine, fetal brain and colon. Gremlin / GREM-1 interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Gremlin may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Gremlin down-regulates the BMP4 signaling in a dose-dependent manner and acts as inhibitor of monocyte chemotaxis.

 

References :

(1) McMahon R., et al., 2000, J. Biol. Chem. 275:9901-9904.
(2) Topol L.Z., et al., 2000, Cytogenet. Cell Genet. 89:79-84.
(3) Jaeger E., et al., 2012, Nat. Genet. 44:699-703.