Human GM-CSF/CSF2 ELISA Kit

分子 : CSF2

 

抗原貨號 : 10015-H07H

 

產品介绍 : For the quantitative determination of Human GM-CSF/CSF2 concentration in cell culture supernates.

 

線性範圍: 7.81-500

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 2.04

 

天然樣本測定含量: Human peripheral blood mononuclear cells （1 x 10E6 cells/mL） were cultured in RPMI supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate, stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of natural GM-CSF, and measured 1913.55 pg/mL.

 

特異性 : This assay recognizes both recombinant and natural Human GM-CSF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human GM-CSF control were assayed for interference. No significant interference was observed.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution