Human Glypican 2 / GPC2 Protein, His Tag

Source : 

Human Glypican 2, His Tag (GP2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gly 554 (Accession # Q8N158-1).

 

Molecule : Glypican 2

 

Synonyms : Glypican 2,GPC2

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_689955.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 60.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.0 kDa. The protein migrates as 55-120 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

 

References :

(1) Herndon ME, et al. 1999. Glycobiology. 9(2):143-55.
(2) Li N, et al. 2017. Proc Natl Acad Sci U S A. 114(32):E6623-E6631.
(3) Bosse KR, et al. 2017. Cancer Cell. 32(3):295-309.