Human Glypican 1 / GPC1 Protein, His Tag

Source : 

Human Glypican 1, His Tag (GP1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1).

 

Molecule : Glypican 1

 

Synonyms : Glypican 1,GPC1

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_002072.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 58.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.0 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH7.5

 

Description :

Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

 

References :

(1) Vermeesch JR, et al. 1995. Genomics. 25(1):327-9.
(2) Ronca F, et al. 2001. J Biol Chem. 276(31):29141-7.
(3) Taylor DR, et al. 2009. PLoS Pathog. 5(11):e1000666.