Human GDF-15 / MIC-1 Protein, His Tag

Source : 

Human GDF-15, His Tag (GD5-H5149) is expressed from E.coli cells. It contains AA Ala 197 - Ile 308 (Accession # Q99988-1).

 

Molecule : GDF-15

 

Synonyms : GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_004855.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 14.2 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.2 kDa. As a result of glycosylation, the protein migrates as 16-17 kDa under reducing (R) condition, and 27 kDa under non-reducing (NR) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 60% ACN, 0.085% TFA

 

Description :

Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1). Expression of MIC-1 mRNA in monocytoid cells is up-regulated by a variety of stimuli associated with activation, including interleukin 1β, tumor necrosis factor α (TNF-α), interleukin 2, and macrophage colony-stimulating factor but not interferon γ, or lipopolysaccharide (LPS). It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. Increased GDF-15 level is linked with the progression and prognosis of the disease condition.

 

References :

(1) Bootcov MR., et al. 1997, Proc Natl Acad Sci U S A., 94(21), 11514-9.
(2) Adela R., et al. 2015, J Diabetes Res., 2015, 490842.