Human GAPDH / G3PDH Protein, His Tag

Source : 

Human GAPDH, His Tag (GAH-H5145) is expressed from E.coli cells. It contains AA Gly 2 - Glu 335 (Accession # AAH01601).

 

Molecule : GAPDH

 

Synonyms : GAPDH,GAPD,CDABP0047,OK/SW-cl.12

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : AAH01601

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 36.9 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.9 kDa. The protein migrates as 37 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH7.5

 

Description :

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is also known as Peptidyl-cysteine S-nitrosylase GAPDH, which is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. GAPDH can participate in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Also, GAPDH can modulate the organization and assembly of the cytoskeleton. Furthermore, GAPDH is a component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.

 

References :

(1) Tokunaga K., et al., 1987, Cancer Res. 47:5616-5619.
(2) Allen R.W., et al., 1987, J. Biol. Chem. 262:649-653.
(3) Jarczowski F., et al., 2009, J. Biol. Chem. 284:766-773.