Human GAPDH Activity Assay Kit II
Kits
- 貨號 Cat. No.
- K2047-100
- 規格 Size
- K2047-100
- Reactivity
- All
- Sample Type
- Animal tissues: Liver 、 Cell culture: Adherent or suspension cells 、 heart etc.
Description
Alternate Name : Glyceraldehyde-3-Phosphate Dehydrogenase,Human GAPDH Activity Kit II,Human GAPDH,GAPDH Activity Assay Kit II,Human GAPDH Activity Kit,Human GAPDH Activity Assay,EC 1.2.1.12,GAPDH Activity Assay,GAPDH Activity Assay Kit,Human GAPDH Activity Assay Kit,GAPDH Assay,GAPDH Activity Kit
Tag Line :
For monitoring GAPDH activity
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
BioVision’s Human GAPDH Activity Assay Kit II provides a simple and sensitive method for monitoring GAPDH activity in various sample types. In this assay, GAPDH catalyzes GAP into BPG and an intermediate, which then reacts with the developer to form a colored product that is measured at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 10 mU/mL in a variety of samples.
Detection Method: Absorbance (OD 450 nm)
Features & Benefits:
Sensitive method for monitoring GAPDH activity in various sample types,Simple, rapid & convenient
Description:
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH is a stable metabolic enzyme and is constitutively expressed. Therefore, it is considered as a housekeeping gene. Deregulation of GAPDH activity is associated with cell proliferation and carcinogenesis. Accurate detection of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. BioVision’s Human GAPDH Activity Assay Kit II provides a simple and sensitive method for monitoring GAPDH activity in various sample types. In this assay, GAPDH catalyzes GAP into BPG and an intermediate, which then reacts with the developer to form a colored product that is measured at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 10 mU/mL in a variety of samples.