Human Fractalkine/CX3CL1 ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

C-X3-C motif chemokine 1; Neurotactin; CX3C membrane-anchored chemokine; Small-inducible cytokine D1

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1329

 

Description (Long Description) : 

The human Fractalkine Kit is a sandwich ELISA. The capture antibody is a polyclonal human Fractalkine antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Fractalkine concentration are added to these wells and allowed to complex with the bound Fractalkine antibody. A biotinylated human Fractalkine polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Fractalkine present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Fractalkine in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen used for this human Fractalkine ELISA is recombinant human Fractalkine amino acids 25-339 that was produced in and purified from a mammalian cell expression system.

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :