Human Flt3 Ligand ELISA Kit (1 Plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Fms-related tyrosine kinase 3 ligand; Flt3 Ligand

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of human Flt3 Ligand in samples such as cell culture supernatants, serum and plasma (heparin, EDTA and Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : Fms-related tyrosine kinase 3 ligand (FLT3LG), is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors (Uniprot).

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1684

 

Description (Long Description) : 

The Human FLT3LG ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal FLT3LG antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FLT3LG concentration are added to these wells and allowed to complex with the bound n FLT3LG antibody. A biotinylated FLT3LG goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FLT3LG present in the samples. The purpose of this kit is the in-vitro quantitative determination of Human FLT3LG in samples such as cell culture supernatants, serum and plasma (heparin, EDTA and Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant human Flt3 Ligand expression system sf21; Immunogen sequence: T27-P185

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, human Flt3 Ligand protein standards, human Flt3 Ligand biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1684