Human FGF4 ELISA Kit (2 Plates) discontinued

ZZZZ_Status (Active, Disc) : Discontinued

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Fibroblast growth factor 4; FGF4

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of human FGF4 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : FGF4 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiations. FGF4 is required for normal limb and cardic valve development during embryogensis (Uniprot).

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1681

 

Description (Long Description) : 

The Human FGF4 Enzyme-Linked Immunosorbent Assay kit (ELISA), is a sandwich ELISA. The capture antibody is a mouse monoclonal FGF4 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FGF4 concentration are added to these wells and allowed to complex with the bound FGF4 antibody. A biotinylated FGF4 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FGF4 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human FGF4 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant Human FGF4 produced in E.coli cells; Immunogen sequence: A31-L206

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plate, human FGF4 protein standards, human FGF4 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1681