Human FGF1 ELISA Kit (2 Plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Fibroblast growth factor 1 (FGF1)

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of Human FGF1 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : FGF1 is important in cell survival, cell division, angigenesis, cell differentiation and cell migration.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1679

 

Description (Long Description) : 

The Human FGF1 ELISA is a solid phase immunoassay specifically designed to measure Human FGF1. The capture antibody is a mouse monoclonal FGF1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FGF1 concentration are added to these wells and allowed to complex with the bound FGF1 antibody. A biotinylated FGF1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FGF1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human FGF1 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant Human FGF1 produced in E.coli cells; Immunogen sequence: A2-D155.

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plate, human FGF1 protein standards, human FGF1 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1679