human FGF-9

SOURCE: E. coli

PURITY: >95% by SDS-PAGE and HPLC Endotoxin level is <0.1 ng per ug of FGF-9.

MOL. WEIGHT: 23.4 kDa

FORM: Lyophilized from 10 mM Tris, pH 7.8 + Ammmonium Sulfate.

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in 10 mM Tris (pH 8.0) to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and store at 4oC for 1 week or stored at -20oC for future use.

STORAGE CONDITIONS: The lyophilized FGF-9 is best-stored desiccated below 0oC. Reconstituted FGF-9 should be stored at 4oC or lyophilized at working aliquots. Repeated freeze/thaw cycles will result in some loss of activity.

FGF-9 (also known as Glial Activating Factor) is a heparin binding growth factor that stimulates the proliferation and activation of glial cells and other cells that express FGF receptors. The human FGF-9 is a 23.4 kDa protein containing 207 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.5 ng/ml, corresponding to a Specific Activity of >2 x 10⁶ units/mg.

USAGE:

FOR RESEARCH USE ONLY! NOT TO BE USED IN HUMANS.