human FGF-7/KGF

SOURCE: E. coli

PURITY: >98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per ug of FGF-7.

MOL. WEIGHT: 18.9 kDa

FORM: Lyophilized from a 1 mg/ml solution with no additives

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in PBS to a concentration of 0.1-1 ug/ul. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

STORAGE CONDITIONS: The lyophilized FGF-7 is best-stored desiccated below 0oC. Reconstituted FGF-7 should be stored in working aliquots at -20oC.

FGF-7, also known as Keratinocyte Growth Factor (KGF), is a potent mitogen for keratinocytes and epithelial cells. Recombinant human FGF-7/KGF is an 18.9 kDa protein containing 163 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells is <10 ng/ml, corresponding to a Specific Activity of >1 x 10⁵ units/mg.

NOTE: For Research Purpose Only! Not to be used in humans.