human FGF-5

SOURCE: E. coli

PURITY: >95% by SDS-PAGE Endotoxin level is <0.1 ng per ug of FGF-5.

MOL. WEIGHT: 27.6 kDa

FORM: Sterile filtered and lyophilized from PBS, pH 7.2

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in 1X PBS, pH 7.2 to a concentration of 0.5-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Freeze/thaw cycles will result in some loss of activity.

STORAGE CONDITIONS: The lyophilized FGF-5 is best-stored desiccated below 0oC. Reconstituted FGF-5 should be stored in working aliquots at -20oC.

FGF-5 is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. Recombinant human FGF-5 is a 27.6 kDa protein containing 252 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: The ED50 determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.5 ng/ml, corresponding to a Specific Activity of >2 x 10⁶ units/mg.

NOTE: For research use only! Not to be used in human.