human FGF-20

SOURCE: E. coli

PURITY: >90% by SDS-PAGE Endotoxin level is <0.1 ng per ug of FGF-20.

MOL. WEIGHT: 23.2 kDa

FORM: Lyophilized from 20 mM Tris, pH 7.6 + 1.5 M NaCl.

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 to 1.0 ug/ul. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Repeated freeze/thaw cycles will result in loss of some activity.

STORAGE CONDITIONS: The lyophilized FGF-20 is best-stored desiccated below 0oC. Reconstituted FGF-20 should be stored at working aliquots at -20oC.

Fibroblast Growth Factor-20 (FGF-20) is a secreted heparin binding growth factor belonging to the FGF family. FGF-20 signals through the FGFR 2c and 3c and is expressed during limb and brian development. Recombinant human FGF-20 is an 23.2 kDa protein containing 209 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is <10 ng/ml, corresponding to a Specific Activity of >1 x 10⁵ units/mg.

USAGE: For Research Purpose Only! Not to be used in humans.