human FGF-2 (bFGF)

SOURCE: E. coli

PURITY: >95% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per ug of FGF-2.

MOL. WEIGHT: 17.2 kDa

FORM: Lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 + 150 mM NaCl

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Repeated freeze/thaw cycles will result in some loss of activity.

STORAGE CONDITIONS: The lyophilized FGF-2 is best-stored desiccated below 0oC. Reconstituted FGF-2 should be stored at working aliquots at -20oC.

FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. The recombinant human FGF-2 is a 17.2 kDa protein containing 154 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.5 ng/ml, corresponding to a Specific Activity of >2 x 10⁶ units/mg.

USAGE: For research use only! Not to be used in human.