human FGF-18

SOURCE: E. coli

PURITY: >95% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per ug of FGF-18.

MOL. WEIGHT: 21.2 kDa

FORM: Lyophilized from 10 mM Tris pH 8.0. The protein may appear as a haze or film, which you may not see at the bottom of the vial.

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris, pH 8.0 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

STORAGE CONDITIONS: The lyophilized FGF-18 is best-stored desiccated below 0oC. Reconstituted FGF-18 should be stored in working aliquots at -20oC. Repeated Freeze/thaw cycles will result in some loss of activity.

The FGFs plays an important role in regulating tissue growth, morphogenesis and tissue repair. FGF-18 is a newly discovered heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. The human FGF-18 is a 21.2 kDa protein containing 182 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.5 ng/ml, corresponding to a Specific Activity of >2 x 10⁶ units/mg.

FOR RESEARCH USE ONLY! NOT TO BE USED IN HUMANS.