human FGF-1

SOURCE: E. coli

PURITY: >98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per ug of FGF-1.

MOL. WEIGHT: 15.8 kDa

FORM: Lyophilized from 1 mg/ml solution of 0.1 M NaCl, 10 mM Tris, pH 7.6.

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in 10 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Repeated freeze/thaw cycles will result in some loss of activity.

STORAGE CONDITIONS: The lyophilized FGF-1 is best-stored desiccated below 0oC. Reconstituted FGF-1 should be stored at -20oC in working aliquots. For long term storage it is recommended to add a carrier protein (e.g., 0.1% BSA).

FGF-1 is an acidic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. Recombinant human FGF-1 is a 15.8 kDa protein containing 140 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <10 ng/ml, corresponding to a Specific Activity of >1 x 10⁵ units/mg.

FOR RESEARCH USE ONLY! NOT TO BE USED IN HUMANS.