Human Fetuin B Protein, His Tag

Source : 

Human Fetuin B, His Tag (FEB-H52H7) is expressed from human 293 cells (HEK293). It contains AA Cys 16 - Pro 382 (Accession # AAH69670 ).

 

Molecule : Fetuin B

 

Synonyms : FETUB,Fetuin-B,16G2,Gugu

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : AAH69670

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 42.4 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 42.4 kDa. The protein migrates as 53-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH8.0

 

Description :

Fetuin-B (FETUB), a second member of the fetuin family in mammals, is also known as Fetuin-like protein IRL685, 16G2 and Gugu, which is expressed in liver and testis and contains 2 cystatin fetuin-B-type domains. FETUB is a protease inhibitor required for egg fertilization. FETUB is also required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity). As a liver-derived plasma protein, FETUB is essential for fertilization.

 

References :

(1) Olivier E., et al., 2000, Biochem. J. 350:589-597.
(2) Dietzel E., et al., 2013, Dev. Cell 25:106-112.
(3) Muzny D.M., et al., 2006, Nature 440:1194-1198.