Human FAP Protein, Fc Tag

Source : 

Human FAP, Fc Tag (FAP-H5263) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).

 

Molecule : FAP

 

Synonyms : FAP,FAPalpha,SIMP,Seprase,APCE

 

Format : Powder

 

Category : Enzymes

 

Accession : N/A

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 111.5 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 111.5 kDa. The protein migrates as 110-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

FAP (also known as seprase) is a Type II transmembrane serine protease. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Degrade also gelatin, heat-denatured type I collagen. Also has dipeptidyl peptidase activity, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

 

References :

(1) Lee KN, et al. Blood. 2004. 103(10):3783-8.
(2) Edosada CY, et al. J Biol Chem. 2006. 281(11):7437-44.
(3) Artym VV, et al. Carcinogenesis. 2002. 23(10):1593-601.