Human Factor X

Prepared from fresh frozen human plasma. The purity of this Vitamin K dependent protein is determined by SDS-PAGE  and shows total reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay. Human Factor X, once activated via the Contact Factor Pathway or the Tissue Factor Pathway, is responsible for the conversion of Prothrombin to Thrombin.

Buffer composition 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4

*Extinction Coefficient (1%) =11.6

One unit =8 µg

Molecular Weight =58,800 daltons