Human Factor VIIa

Prepared from purified Human Factor VII using Human Factor XIIa. The Factor Xlla is removed using our CTI column. Complete activation is observed on a 10% SDS-PAGE gel. The clotting activity is greater than 30,000 u/mg. This protein is a single band at 50,000 MW on a non-reduced gel and reduces to 29,500 and 23,500 with the addition of 2-mercaptoethanol. Factor Vlla, in the presence of calcium ions and Tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4 *Extinction Coefficient (1%) = 13.9 Molecular Weight = 50,000 daltons.