Human Factor V

Prepared from fresh frozen human plasma. Human Factor V is a single chain glycoprotein with a MW of 330,000. Human Factor V is activated by thrombin and less effectively by Factor Xa to yield the active cofactor Va. Human Factor Va, Factor Xa, and phospholipid form the prothrombinase complex which is responsible for the rapid activation of Prothrombin to Thrombin. Human Factor V is homogeneous as judged by a 4-20% gradient gel and shows no reduction upon incubation with 2-mercaptoethanol. Buffer =10 mM Tris-HCl/10 mM Boric Acid/1 mM CaCl2 /50% Glycerol/pH 7.2 *Extinction Coefficient (1%) = 9.6 Molecular Weight = 330,000 daltons.