Human ErbB3 / Her3 Protein, Strep Tag

Source : 

Human ErbB3, Strep Tag (ER3-H5288) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Thr 643 (Accession # NP_001973.2).

 

Molecule : ErbB3

 

Synonyms : ERBB3,HER3,LCCS2,MDA-BF-1,MGC88033,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_001973.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 71.7 kDa

 

Characteristics :

This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 71.7 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

 

References :

(1) Singer, E. etal., 2001, J. Biol. Chem. 276 (47): 44266–74
(2) Kraus,M.H.etal.,1989,Proc.Natl.Acad.Sci.86:9193-9197.
(3) Carraway,K.L.etal.,1994,J.Biol.Chem.269:14303-14306.
(4) Wallasch,C.etal.,1995,EMBO.J.14:4267-4275.
(5) Alimandi,M.etal.,1995,Oncogene.10:1813-1821.
(6) Gamett,D.C.etal.,1995,J.Biol.Chem.270:19022-19027.