Human Ephrin-A1 / EFNA1 ELISA Kit

分子 : EFNA1

 

抗原貨號 : 10882-H08H

 

產品介绍 : For the quantitative determination of Human Ephrin-A1 / EFNA1 concentration in cell culture supernates.

 

線性範圍: 23.44-1500

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : rabbit mAb

 

靈敏度: 4

 

天然樣本測定含量: Human liver cells (HepG2) were grown to confluency in DMEM supplemented with 10% fetal
calf serum,Collected the supernate and assayed for
natural levels of EFNA1.The concentration of Human EFNA1 detected in a HepG2 cell culture supernates is 115pg/mL.

 

特異性 : This assay recognizes both recombinant and natural Human EFNA1.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of EphA2、EphA3、EphA4 and EphA7 at 15 ng/mL in a mid-range recombinant human EFNA1 control were assayed for interference. No significant interference was observed.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution