Human EphA4 Protein, His Tag

Source : 

Human EphA4, His Tag (EP4-H52H8) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Thr 547 (Accession # P54764-1).

 

Molecule : EphA4

 

Synonyms : EphA4,HEK8,SEK,TYRO1,EK8

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_004429.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 60.3 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

 

References :

(1) Fu WY, et al. 2007. Nat Neurosci. 10(1):67-76.
(2) Miao H, et al. 2009. Cancer Cell. 16(1):9-20.
(3) Zhang G, et al. 2008. Cancer Res. 68(6):1691-6.