Human ENPP-2 / Autotaxin Protein, His Tag

Source : 

Human ENPP-2, His Tag (EN2-H5241) is expressed from human 293 cells (HEK293). It contains AA Asp 49 - Ile 863 (Accession # NP_001035181).

 

Molecule : ENPP-2

 

Synonyms : ENPP-2,E-NPP 2,Autotaxin,LysoPLD,ATX,PDNP2,ATX-X,NPP2,PD-IALPHA

 

Format : Powder

 

Category : Enzymes

 

Accession : NP_001035181

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 94.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 94.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH 7.5

 

Description :

Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.

 

References :

(1) Stracke M.L., et al., 1992, J. Biol. Chem. 267:2524-2529.
(2) Nam S.W., et al., 2001, Cancer Res. 61:6938-6944.
(3) Hausmann J., et al., 2011, Nat. Struct. Mol. Biol. 18:198-204.