Human DNAM-1 / CD226 Protein, Fc Tag

Source : 

Human DNAM-1, Fc Tag (DN1-H5257) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557).

 

Molecule : DNAM-1

 

Synonyms : DNAM1,CD226,PTA1

 

Format : Powder

 

Category : Immune Checkpoint Proteins

 

Accession : NP_006557

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 52.6 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.6 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

 

References :

(1) Shibuya A., et al.,1995, Immunity 4:573-581.
(2) Tahara-Hanaoka S., et al .,2003, Int. Immunol. 16:533-538.
(3) Pende D., et al.,2004, Mol. Immunol. 42:463-469.