Human Dkk-3 (R335G) Protein, His Tag

Source : 

Human Dkk-3 (R335G), His Tag (DK3-H5220) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Ile 350 (Accession # Q9UBP4-1 (R335G)).

 

Molecule : Dkk-3

 

Synonyms : DKK3,REIC,RIG

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_001018067.1 (R335G)

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 37.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.0 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.

 

References :

(1) Medinger M, Tzankov A,et al., 2011, Thromb Haemost. 105(1):72-80.
(2) Yue W, Sun Q, et al., 2008, Carcinogenesis. 29(1):84-92..
(3) Pinho S, Niehrs C.et al., 2007, Differentiation. 75(10):957-67.