Human Cystatin S / CST4 Protein, His Tag

Source : 

Human Cystatin S, His Tag (CS4-H5223) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ala 141 (Accession # AAH65714).

 

Molecule : Cystatin S

 

Synonyms : CST4,Cystatin-4,Cystatin-S,Cystatin-S5,Cystatin-SA-III

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : AAH65714

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 15.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.0 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 25 mM MES, 150 mM NaCl, pH6.0

 

Description :

Cystatin-4 (CST4) is also known as Cystatin-S, Salivary acidic protein 1, which is a secreted protein, which belongs to the cystatin family. Cystatin-S is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) and is also expressed in saliva, tears, urine and seminal fluid. Cystatin-4 / Cystatin S strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.

 

References :

(1) Lamkin M.S., et al., 1991, Arch. Biochem. Biophys. 288:664-670.
(2) Ryan C.M., et al., 2010, J. Am. Soc. Mass Spectrom. 21:908-917.
(3) Isemura S., et al., 1991, J. Biochem. 110:648-654.