Human Cystatin C / CST3 ELISA Kit

分子 : CST3

 

抗原貨號 : 10439-H08H

 

產品介绍 : For the quantitative determination of Human Cystatin C / CST3 concentration in serum, cell culture supernates, and urine.

 

線性範圍: 3.91-250

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 1.54

 

天然樣本測定含量: The average concentration of CST3 in 10 normal human serum is 66.98 +/- 24.39 ng/mL ranging from 41.39 to 113.36 ng/mL. The average concentration of CST3 in 10 normal human urine is 4.30 +/- 2.50 ng/mL ranging from 0.67 to 9.50 ng/mL. HepG2 cells (0.2 x 10E5 cells/mL) were cultured 3 days. An aliquot of the cell culture supernate was removed, assayed for levels of natural CST3, and measured 15.58 ng/mL.

 

特異性 : This assay recognizes both recombinant and natural Human CST3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 :

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution