Human Cyclooxygenase-2 (Cox-2) ELISA Kit

SpeciesReactivity

Human

Samples

culture media, cells or tissue extracts. It is not recommended for serum or plasma due to low concn of Cox-2

KitContents

1. Anti-Human Cox-2 antibody coated strip plate (96 wells), Cat. #100200-1 1 plate 2. Anti-human Cox-2-HRP conjugate, powder 3. Cat # 100200-2 (reconstitute in 10.5 ml conjugate diluent) 1 vial 4. Cox-2 ELISA Buffer, Cat # 100200-3 (30 ml) 1 bottle 5. Purified recombinant Human Cox-2 Standard Stock (550 ng/ml; see vial for exact concn (Powder) reconstitute in .5 ml distilled water; Cat # 100200-4 1 Vial 6. Chromogen (TMB); Cat # 100200-5, 15 ml 1 bottle 7. Stop Solution (1N H2SO4) 8. cat # 100200-6 (11 ml) 1 bottle 9. Wash Buffer (40X) Dilute 1:40 before use 10. Cat # 100200-7 (50 mls) 1 bottle 11. Conjugate Diluent, cat # 100200-8 (10.5 ml) 1 bottle 12. Instruction Manual, 100-200-CX2 1

Form

Stds=0-275 ng/ml ; Sample=100 ul; Sensitivity= 2 ng/ml; 120 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

The antibodies used in this kit are specific for Cox-2. No significant reactivity is seen with Cox-1. Suitability of the human Cox-2 kit with other species (Mouse rat Cox-2) has not been determined.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates