Human CXADR / CAR Protein, His Tag

Source : 

Human CXADR, His Tag (CXR-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Gly 237 (Accession # NP_001329.1).

 

Molecule : CXADR

 

Synonyms : CXADR,CAR,CAR4/6,HCAR

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_001329.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 24.9 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24.9 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Coxsackie virus and adenovirus receptor (CXADR) is also known as CAR, is a type I transmembrane glycoprotein for group B coxsackie viruses and subgroup C adenoviruses, and belongs to the CTX family of the Ig superfamily. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins such as: fibronectin, agrin, laminin-1 and tenascin-R. Human CXADR protein contains a signal sequence, a extracellular domain (ECD) with a V­ type (D1) and a C2­ type (D2) Ig­like domain, a  transmembrane segment and a intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions, and is necessary and sufficient for adenovirus binding. Variants of CXADR are attached to the cell membrane by a GPI- anchor.

 

References :

(1) Bergelson JM, et al., 1997, Science 275 (5304): 1320–3.
(2) Tomko RP, et al., 1997, Proc Natl Acad Sci U S A 94 (7): 3352–6.
(3) Patzke C, et al., 2010, Journal of Neuroscience 30 (8): 2897–2910.
(4) Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-80.