Human Cortisol ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Other fluids not optimized.

KitContents

1. Anti-cortisol coated microwell strip plate (96 wells) 1851 2. Cortisol Std. A (0 ug/dl), 0.5 ml 1852A 3. Cortisol Std. B (1 ug/dl), 0.5 ml 1852B 4. Cortisol Std. C (3 ug/dl), 0.5 ml 1852C 5. Cortisol Std. D (10 ug/dl), 0.5 ml 1852D 6. Cortisol Std. E (30 ug/dl), 0.5 ml 1852E 7. Cortisol Std. F (60 ug/dl), 0.5 ml 1852F 8. Cortisol-HRP Conjugate; 11 ml 1853 9. Wash buffer (20X), 50 ml (dilute 1:20 with water) W-20 10. HRP substrate Solution A; 11 ml 1850SA 11. HRP substrate Solution B; 11 ml 1850SB 12. Stop solution, 10 ml T-10 13. Complete Instruction Manual M1850

Form

Stds=0-60 ug/dL ; Sample=10 ul; Sensitivity= 0.36 ug/dL; 90 min assay; Quantitative, Competitive assay

SpCrossreactivity

The following compounds were tested for crossreactivity of the assay: Cortisol (100%), Corticosterone (9.3%), Cortisone (2.2%) 11-Deoxycorticosterone (0.6%), 11-deoxycortisol-a-hydroxyCortisol (3.6%), Dexamethasone (0.3%), Epiandrosterone (0.0%), 17-a-hydroxyprogesterone (1.0%), Prednisolone (33.3%), Prednisone (1.4%), Progesterone, Testosterone, Estradiol (<0.1%). Cortisol kit has been designed and tested for human serum samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Since the steroid hormone is the same in all species, this kit should work in most species as long as the sample cocn is within the range of this kit.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates