Human Complement Factor D / CFD Protein, Fc Tag

Source : 

Human Complement Factor D, Fc Tag (CFD-H5256) is expressed from human 293 cells (HEK293) and inactive. It contains AA Ile 26 - Ala 253 (Accession # P00746-1).

 

Molecule : Complement Factor D

 

Synonyms : CFD,Adipsin,PFD,DF,Complement factor D

 

Format : Powder

 

Category : Enzymes

 

Accession : NP_001919.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 50.8 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.8 kDa. The protein migrates as 53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Complement factor D (CFD) is also known as Adipsin, C3 convertase activator, Properdin factor D (PFD), which contains one peptidase S1 domain and belongs to the peptidase S1 family. CFD / Adipsin cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. CFD / Adipsin is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement factor D deficiency which predisposes to invasive meningococcal disease.

 

References :

(1) White R.T., et al., 1992, J. Biol. Chem. 267:9210-9213.
(2) Johnson D.M.A., et al., 1984, FEBS Lett. 166:347-351.
(3) Sprong T., et al., 2006, Blood 107:4865-4870.